MSc ii sem forensic toxicology
@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ describe liquid liquid methods of extraction of poison in toxicology In toxicology, liquid-liquid extraction (LLE) is a widely used technique to isolate and concentrate poisons (toxic substances) from biological and environmental samples. This method exploits the differential solubility of compounds in two immiscible liquids, typically water and an organic solvent. Here's a detailed description of the process: Principles of Liquid-Liquid Extraction Partition Coefficient: The basis of LLE is the partition coefficient (K), which is the ratio of the concentrations of a compound in a mixture of two immiscible solvents at equilibrium. The compound will distribute itself between the two solvents based on its affinity for each. Choice of Solvents: The solvents are chosen based on their immiscibility and the solubility of the target poison. Common solvent pairs include water and organic solvents like chloroform, ethyl acetate, or hexane....